This week I had the opportunity to plant my own mini garden (dimensions to follow). Thank you Elena Ortiz, Patty and Leah (my apologies in advance if I botched the spelling on the last two) for allowing me to use some of your limited space. It was actually more time consuming and more fun than what I had anticipated. Matt would be proud, I actually got mud under my fingernails. Below I have attached a garden map so you will be able to see what I have been up to. My plants in the lighting incubator are still growing. I talk to them daily. We have a symbiotic relationship.
One difference with my project this year from last is its breadth. Isolating and identifying Rhizobia offers me a variety of tasks to complete; some in succession while others concurrently. For example, to date, I have poured two different types of media, planted both indoors and out, and continued with my research on Rhizobia. In the very near future, I will be able to take the nodules off a legume root, clean them, crush them, inoculate media with it, grow it, identify it through micro tests I learned in 205, apply it to Mueller Hinton and measure zones of inhibition (one of my favorites) and lastly, run it through Polymerase Chain Reaction (PCR) and electrophoresis. And that is just the beginning... More details to follow...
Yes Jervana and Gilbert, I was jealous of your projects and how they involved PCR and Matt specifically redesigned my entire project to incorporate it.
My nicely tilled, sowed, and watered garden
My garden map
My precious legumes 8-)
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